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EPH Receptor A3 抗体 (Extracellular, N-Term)

The 兔多克隆 anti-EPH Receptor A3 antibody is suitable to detect EPH Receptor A3 in samples from 人. It has been validated for WB, IHC, IF, FACS 和 LCI.

Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 5), human K562 chronic myelogenous leukemia cell line lysate (lanes 2 and 6), human Malme-3M melanoma cell line lysate (lanes 3 and 7) and human HT-29 colorectal adenocarcinoma cell line lysate (lanes 4 and 8): - 1-4. (EPH Receptor A3 抗体 (Extracellular, N-Term))

Expression of EphA3 in rat and mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections with Anti-EphA3 (extracellular) Antibody (ABIN7043123, ABIN7044245 and ABIN7044246), (1:200), followed by goat anti-rabbit-AlexaFluor-488. (EPH Receptor A3 抗体 (Extracellular, N-Term))

IHC

Cell surface detection of EphA3 in live intact human Jurkat T-cell leukemia cells: (black line) Cells. (EPH Receptor A3 抗体 (Extracellular, N-Term))

FACS

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. (EPH Receptor A3 抗体 (Extracellular, N-Term))

4 images

产品编号 ABIN7043123

发货至: 中国

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Quick Overview for EPH Receptor A3 抗体 (Extracellular, N-Term) (ABIN7043123)

抗原

See all EPH Receptor A3 (EPHA3) 抗体

EPH Receptor A3 (EPHA3)

适用

人

宿主

兔

克隆类型

多克隆

标记

This EPH Receptor A3 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Discover our top product EPHA3 Primary Antibody

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AA 488-500, Extracellular, N-Term

原理

A Rabbit Polyclonal Antibody to EphA3 Receptor

交叉反应

人, 小鼠, 大鼠

预测反应

Rat,mouse - identical

产品特性

Anti-EphA3 (extracellular) Antibody (ABIN7043123, ABIN7044245 and ABIN7044246) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize EphA3 from human, rat, and mouse samples.

纯化方法

Affinity purified on immobilized antigen.

免疫原

(C)RARGTNVTISSLK, corresponding to amino acid residues 488-500 of human EphA3

亚型

IgG
anti-EPH Receptor A3 (EPHA3) (AA 202-326) antibody
EPHA3 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 3A12 unconjugated

anti-EPH Receptor A3 (EPHA3) (AA 896-928), (C-Term) antibody
EPHA3 适用: 人, Hamster WB, IHC (p) 宿主: 兔 Polyclonal RB1585 unconjugated

anti-EPH Receptor A3 (EPHA3) (AA 896-928) antibody
EPHA3 适用: 人, Hamster WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-EPH Receptor A3 (EPHA3) (AA 202-326) antibody
EPHA3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E9 unconjugated

anti-EPH Receptor A3 (EPHA3) (C-Term) antibody
EPHA3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-EPH Receptor A3 (EPHA3) (AA 38-375) antibody
EPHA3 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-EPH Receptor A3 (EPHA3) (AA 751-983) antibody
EPHA3 适用: 人 ELISA 宿主: 小鼠 Monoclonal 6C1B6 unconjugated

anti-EPH Receptor A3 (EPHA3) (AA 115-144), (N-Term) antibody
EPHA3 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB01584 unconjugated

anti-EPH Receptor A3 (EPHA3) (AA 115-144) antibody
EPHA3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-EPH Receptor A3 (EPHA3) (AA 401-500) antibody
EPHA3 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:200
FC: 5 μg per reaction
ICC: The optimal concentration should be determined by the user
IHC: 1:200
IP: The optimal concentration should be determined by the user

说明

Negative Control: (ABIN7235282)
Blocking Peptide: (ABIN7235282)

限制

仅限研究用
状态

Lyophilized

溶解方式

25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

浓度

0.8 mg/mL

缓冲液

PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
抗原

EPH Receptor A3 (EPHA3)

别名

EPHA3

背景

Synonyms: Ephrin type-A receptor 3, EPH-like kinase 4, EK4, Tyrosine-protein kinase TYRO4
Description: Eph receptors are the largest family of receptor tyrosine kinases (RTKs). Based on sequence similarity and the type of ephrin ligands that they bind, they are classified into A and B sub-classes. EphA receptors bind ephrin-A ligand while EphB bind ephrin-B ligand.Eph receptors play an important role during embryonic development and are involved in several processes including cell adhesion, cell migration, and tissue boundary formation. Eph receptors are typically expressed at low levels in normal adult tissues1,2.EphA3 (Ephrin type-A receptor 3) structure includes a ligand-binding domain (LBD) at the N-terminus followed by a cysteine-rich region and two fibronectin III repeats. The receptor-binding domain of the ephrins is connected through a short linker segment to a GPI-anchor or a transmembrane segment3.EphA3 was first identified as a surface antigen on pre-B lymphoblastic leukemia cells. High EphA3 levels have been detected in a number of hematological cancers and solid tumors such as lung cancers, melanomas, gastric carcinoma, leukemia, in B and T cell malignancies and in glioblastoma multiforme. In addition, high levels of EphA3 are implicated in lymph node metastasis, advanced stages of colorectal cancer, and prostate cancer1,3,4.

基因ID

2042

NCBI登录号

NM_005233

UniProt

P29320

途径

RTK signaling, Regulation of Cell Size