CCR2 抗体 (Extracellular)
Quick Overview for CCR2 抗体 (Extracellular) (ABIN7043032)
抗原
See all CCR2 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 11-25, Extracellular
-
原理
- A Rabbit Polyclonal Antibody to CCR2 Receptor
-
特异性
- Extracellular, N-terminus
-
交叉反应 （详细）
- The antibody will not recognize CCR2 from mouse and rat samples.
-
预测反应
- Human only
-
产品特性
- Anti-Human CCR2 (extracellular) Antibody (ABIN7043032, ABIN7044170 and ABIN7044171) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, indirect live cell flow cytometry applications. It has been designed to recognize CCR2 from human samples only. The antibody will not recognize CCR2 from mouse and rat samples.
-
纯化方法
- Affinity purified on immobilized antigen.
-
免疫原
-
Immunogen: Synthetic peptide
Immunogen Sequence: RNTNESGEEVTTFFD(C), corresponding to amino acid residues 11-25 of human CCR2
-
亚型
- IgG
-
-
anti-Chemokine (C-C Motif) Receptor 2 (CCR2) antibody
CCR2 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 3B6B1 unconjugated
anti-Chemokine (C-C Motif) Receptor 2 (CCR2) (AA 301-373) antibodyCCR2 适用: 人, 小鼠, 大鼠 ELISA, FACS, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 2 (CCR2) antibodyCCR2 适用: 人, 小鼠, 大鼠, 犬 IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 2 (CCR2) (N-Term) antibodyCCR2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 2 (CCR2) (N-Term) antibodyCCR2 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 2 (CCR2) (AA 1-42) antibodyCCR2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4D12 unconjugated
anti-Chemokine (C-C Motif) Receptor 2 (CCR2) (AA 315-374) antibodyCCR2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 2 (CCR2) antibodyCCR2 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 2 (CCR2) antibodyCCR2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 2 (CCR2) antibody (HRP)CCR2 适用: 人 WB, IHC 宿主: 兔 Polyclonal HRP
-
-
应用备注
-
Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:1500
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
-
浓度
- 1 mg/mL
-
缓冲液
- PBS pH 7.4
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
-
-
- CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))
-
别名
- CCR2
-
背景
-
C-C chemokine receptor type 2, Monocyte chemoattractant protein 1 receptor, MCP-1 receptor, CC-CKR-2, CD192,CC chemokine receptor 2 (CCR2) is one of 19 members of the chemokine receptor subfamily of class A G-protein-coupled receptors (GPCRs). CCR2 is expressed in monocytes, immature dendritic cells, and T-cell subpopulations, and mediates acute inflammation by driving leukocyte migration to damaged or infected tissues towards chemokine ligands such as CCL2. Elevated expression of chemokines and their receptors such as CCR2 and its ligands can contribute to chronic inflammation and malignancy and hence these molecules are implicated in numerous inflammatory and neurodegenerative diseases including atherosclerosis, multiple sclerosis, asthma, neuropathic pain, and diabetic nephropathy, as well as cancer. These disease associations have motivated numerous studies in search of therapies that target the CCR2-chemokine axis1,2.As with most G-protein coupled receptors (GPCRs), chemokine receptors transmit signals across cell membranes by means of extracellular ligand and intracellular G-protein binding. CCR2 has seven transmembrane alpha helixes, and can take distinct conformational states that are necessary for chemokine/ligand binding, G-protein binding, activation, inactivation, and signal transmission3.CCR2 can form homo- or heterodimers with other chemokine receptors. Homodimerization may be necessary for CCR2 chemotactic activity and occurs in the absence of the ligand. CCR2/CCR5 heterocomplexes activate calcium response and support cell adhesion rather than chemotaxis, whereas CCR2/CCR4 heterodimers have an allosteric trans-inhibitory effect on CCL2 binding2.
Alternative names: C-C chemokine receptor type 2, Monocyte chemoattractant protein 1 receptor, MCP-1 receptor, CC-CKR-2, CCR2, CD192 -
基因ID
- 729230
-
NCBI登录号
- NM_000647
-
UniProt
- P41597
-
途径
- cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
-
