Calnexin 抗体 (Intracellular) (Atto 594)
Quick Overview for Calnexin 抗体 (Intracellular) (Atto 594) (ABIN7043022)
抗原
See all Calnexin (CANX) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 572-587, Intracellular
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原理
- A Rabbit Polyclonal Antibody to Calnexin Conjugated to the Fluorescent Dye ATTO-594
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特异性
- Intracellular, C-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse,human - identical
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产品特性
- Anti-Calnexin Antibody (ABIN7043021, ABIN7044212 and ABIN7044213) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Canx from human, mouse, and rat samples. \nAnti-Calnexin-ATTO Fluor-594 Antibody (ABIN7043022) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Calnexin-ATTO Fluor-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)KAEEDEILNRSPRNRK, corresponding to amino acid residues 572-587 of rat Canx
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亚型
- IgG
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anti-Calnexin (CANX) (C-Term) antibody
CANX 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 兔, Pig, 猴, Hamster, 绵羊, 小鸡, Avian, 马, 山羊, Cat, 驴 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-Calnexin (CANX) (Internal Region) antibodyCANX 适用: 人, 小鼠, 大鼠 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Calnexin (CANX) (C-Term) antibodyCANX 适用: 犬 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calnexin (CANX) (AA 263-482) antibodyCANX 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calnexin (CANX) (AA 504-592) antibodyCANX 适用: 人 WB, IF, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Calnexin (CANX) (AA 1-300), (N-Term) antibodyCANX 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal CANX-1541 unconjugated
anti-Calnexin (CANX) (AA 239-461) antibodyCANX 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calnexin (CANX) (AA 239-461) antibodyCANX 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C8 unconjugated
anti-Calnexin (CANX) (AA 1-300), (N-Term) antibodyCANX 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal CANX-1543 unconjugated
anti-Calnexin (CANX) (C-Term) antibodyCANX 适用: 犬 WB, IHC, IF, IP, FACS, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:60
Application Dilutions Western blot wb: N/A
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说明
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Negative Control: (ABIN7235025)
Blocking Peptide: (ABIN7235025)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 50 μL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Calnexin (CANX)
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别名
- CANX
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背景
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Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90,Calnexin is a type I transmembrane endoplasmic reticulum chaperone protein involved in the folding and assembly of numerous proteins. Calnexin is comprised of 537 amino acids with a large luminal domain consisting of 462 amino acids. Calnexin does not possess N-linked glycosylation sites and is localized to the endoplasmic reticulum by a -RKPRRE motif at its carboxyl terminus. Calnexin is one of the few ER membrane proteins that can be phosphorylated in a GTP-dependent manner1.Calnexin interacts with a number of ER membrane channels. It interacts transiently with wild-type Shaker K+ channel in the ER but fails to associate with an unglycosylated Shaker mutant that makes active, cell surface channels. This suggest that glycosylation of shaker protein is required for interaction with calnexin but calnexin is not required for the proper assembly and folding of Shaker channels2.Coexpression of Calnexin with the voltage-gated potassium channel KV1.2 was found to produce a substantial dose-dependent increase in cell surface KV1.2 channels in transfected mammalian COS-1 cells. In contrast, Calnexin coexpression showed no effect on trafficking of intracellularly retained KV1.1 or KV1.6 subunits3.Calnexin has been implicated in the pathophysiology of cystic fibrosis disease (CF). Wild type and ΔF508 mutant cystic-fibrosis conductance regulator (CFTR) associate with Calnexin in a Chinese hamster ovary (CHO) cell model. Although both wild-type and ΔF508 CFTRs are present in complexes with Calnexin, only wild-type CFTR is able to escape this association and exit the ER. This suggests that Calnexin retains misfolded proteins in the ER and contributes to the mislocalization of mutant CFTRs.
Alternative names: Calnexin, Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90 -
基因ID
- 29144
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NCBI登录号
- NM_001024649
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UniProt
- P35565
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途径
- MAPK Pathway, Thyroid Hormone Synthesis
抗原
-
