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Calnexin 抗体 (Intracellular)

Name: Calnexin 抗体 (Intracellular)
SKU: ABIN7043021
Availability: OutOfStock

This anti-Calnexin antibody is a 兔多克隆 antibody detecting Calnexin in WB, IHC 和 IF. Suitable for 大鼠.

Expression of Calnexin in mouse hippocampus - Immunohistochemical staining of mouse hippocampal CA1 region using Anti-Calnexin Antibody (ABIN7043021, ABIN7044212 and ABIN7044213), (1:200). (Calnexin 抗体 (Intracellular))

IHC

Western blot analysis of rat heart membrane (lanes 1 and 5), mouse heart membrane (lanes 2 and 6), human breast adenocarcinoma (MCF7) lysate (lanes 3 and 7) and human ovary adenocarcinoma (OVCAR-3) cell line lysate (lanes 4 and 8): - 1-4. (Calnexin 抗体 (Intracellular))

2 images

产品编号 ABIN7043021

发货至: 中国

Quick Overview for Calnexin 抗体 (Intracellular) (ABIN7043021)

抗原

See all Calnexin (CANX) 抗体

Calnexin (CANX)

适用

大鼠

宿主

兔

克隆类型

多克隆

标记

This Calnexin antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Discover our top product CANX Primary Antibody

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AA 572-587, Intracellular

原理

A Rabbit Polyclonal Antibody to Calnexin

特异性

Intracellular, C-terminus

交叉反应

人, 小鼠, 大鼠

预测反应

Mouse,human - identical

产品特性

Anti-Calnexin Antibody (ABIN7043021, ABIN7044212 and ABIN7044213) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Canx from human, mouse, and rat samples.

纯化方法

Affinity purified on immobilized antigen.

免疫原

Immunogen: Synthetic peptide
Immunogen Sequence: (C)KAEEDEILNRSPRNRK, corresponding to amino acid residues 572-587 of rat Canx

亚型

IgG
anti-Calnexin (CANX) (C-Term) antibody
CANX 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 兔, Pig, 猴, Hamster, 绵羊, 小鸡, Avian, 马, 山羊, Cat, 驴 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated

anti-Calnexin (CANX) (Internal Region) antibody
CANX 适用: 人, 小鼠, 大鼠 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Calnexin (CANX) (C-Term) antibody
CANX 适用: 犬 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Calnexin (CANX) (AA 263-482) antibody
CANX 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Calnexin (CANX) (AA 504-592) antibody
CANX 适用: 人 WB, IF, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 1D4 unconjugated

anti-Calnexin (CANX) (AA 1-300), (N-Term) antibody
CANX 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal CANX-1541 unconjugated

anti-Calnexin (CANX) (AA 239-461) antibody
CANX 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Calnexin (CANX) (AA 239-461) antibody
CANX 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C8 unconjugated

anti-Calnexin (CANX) (AA 1-300), (N-Term) antibody
CANX 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal CANX-1543 unconjugated

anti-Calnexin (CANX) (C-Term) antibody
CANX 适用: 犬 WB, IHC, IF, IP, FACS, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200
Application Dilutions Western blot wb: 1:400-1:600

说明

Negative Control: (ABIN7235025)
Blocking Peptide: (ABIN7235025)

限制

仅限研究用
状态

Lyophilized

溶解方式

0.2 mL double distilled water (DDW).

浓度

1 mg/mL

缓冲液

PBS pH 7.4

储存条件

4 °C,-20 °C

储存方法

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
抗原

Calnexin (CANX)

别名

CANX

背景

Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90,Calnexin is a type I transmembrane endoplasmic reticulum chaperone protein involved in the folding and assembly of numerous proteins. Calnexin is comprised of 537 amino acids with a large luminal domain consisting of 462 amino acids. Calnexin does not possess N-linked glycosylation sites and is localized to the endoplasmic reticulum by a -RKPRRE motif at its carboxyl terminus. Calnexin is one of the few ER membrane proteins that can be phosphorylated in a GTP-dependent manner1.Calnexin interacts with a number of ER membrane channels. It interacts transiently with wild-type Shaker K+ channel in the ER but fails to associate with an unglycosylated Shaker mutant that makes active, cell surface channels. This suggest that glycosylation of Shaker protein is required for interaction with calnexin but calnexin is not required for the proper assembly and folding of Shaker channels2.Coexpression of calnexin with the voltage-gated potassium channel KV1.2α was found to produce a substantial dose-dependent increase in cell surface KV1.2α channels in transfected mammalian COS-1 cells. In contrast, calnexin coexpression showed no effect on trafficking of intracellularly retained KV1.1 or KV1.6α subunits3.Calnexin has been implicated in the pathophysiology of cystic fibrosis disease (CF). Wild type and ΔF508 mutant cystic-fibrosis conductance regulator (CFTR) associate with calnexin in a Chinese hamster ovary (CHO) cell model. Although both wild-type and ΔF508 CFTRs are present in complexes with calnexin, only wild-type CFTR is able to escape this association and exit the ER. This suggests that calnexin retains misfolded proteins in the ER and contributes to the mislocalization of mutant CFTRs.

Alternative names: Calnexin, Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90

基因ID

29144

NCBI登录号

NM_001024649

UniProt

P35565

途径

MAPK Pathway, Thyroid Hormone Synthesis