CACNA1B 抗体 (Intracellular)
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- 抗原 See all CACNA1B 抗体
- CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))
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抗原表位
- AA 851-867, Intracellular
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CACNA1B antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunochromatography (IC)
- 原理
- A Rabbit Polyclonal Antibody to CACNA1B (CaV2.2) Channel
- 特异性
- Intracellular loop between domains II and III
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- rabbit - 11,Mouse - 15,17 amino acid residues identical, human 11
- 产品特性
- Anti-CACNA1B (CaV2.2) Antibody (ABIN7043005, ABIN7043951 and ABIN7043952) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize CaV2.2 from mouse, rat, and human samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 质量等级
- KO Validated
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RHHRHRDRDKTSASTPA, corresponding to amino acid residues 851-867 of rat CACNA1B
- 亚型
- IgG
- Top Product
- Discover our top product CACNA1B Primary Antibody
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anti-Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B) (AA 2132-2339) antibody
CACNA1B 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B) antibodyCACNA1B 适用: 人, 大鼠, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B) (C-Term) antibodyCACNA1B 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B) (AA 1921-2222) antibodyCACNA1B 适用: 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B) (alpha-1E subunit) antibodyCACNA1B 适用: 大鼠, 小鼠 WB, ICC 宿主: 小鼠 Monoclonal 62C10 unconjugated
anti-Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B) antibodyCACNA1B 适用: 大鼠, 小鼠 WB, IHC 宿主: 豚鼠 Polyclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100
Application Dilutions Western blot wb: 1:200
- 说明
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Cited Application: IP|IHC
Negative Control: (ABIN7234980)
Blocking Peptide: (ABIN7234980)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- 0.2 mL double distilled water (DDW).
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))
- 别名
- CACNA1B (CACNA1B 产品)
- 背景
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Voltage-dependent N-type calcium channel subunit α1B, Brain calcium channel III, BIII,Voltage-dependent Ca2+ channels (CaV channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.1The voltage-dependent Ca2+ channels are composed of several subunits, α1, β, α2δ and γ. CaV channels were originally divided into six physiological types: L-, N-, P-, Q-, R-, and T-type.The CaV2.2 (formally named α1B) composes the α1 poreforming subunit for the N-type Ca2+ channel family. They are involved in neurotransmitter release from central neurons, including glutamate, γ-aminobutyric acid, acetylcholine, dopamine and noradrenaline.2The CaV2.2 is expressed preferentially in the central nervous system, where along with CaV2.1, it is responsible for pre-synaptic Ca2+ influx and neurotransmitter release.1,3The CaV2.2 channel is negatively regulated by many different GPCRs. There are two ways that this is done: either by directly binding Gβγ to the channel or by an indirect mechanism involving second messenger and channel phosphorylation.4 ω-Conotoxin GVIA (#C-300) is a specific blocker of CaV2.2 Ca2+ channels. It specifically blocks N-type CaV channels by binding to the CaV2.2 α1 subunit (α1B) and its action is only partially reversible.5,6
Alternative names: CACNA1B (CaV2.2), Voltage-dependent N-type calcium channel subunit alpha1B, Brain calcium channel III, BIII - 基因ID
- 257648
- NCBI登录号
- NM_000718
- UniProt
- Q02294
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