BDNF 抗体 (Intracellular)
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Quick Overview for BDNF 抗体 (Intracellular) (ABIN7042970)
抗原
See all BDNF 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 166-178, Intracellular
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原理
- A Rabbit Polyclonal Antibody to BDNF
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交叉反应
- 人, 小鼠, 大鼠
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交叉反应 （详细）
- The antibody is specific for BDNF, it does not crossreact with NGF, NT-3 or NT-4.
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预测反应
- Mouse,rat,many other species - identical
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产品特性
- Anti-BDNF Antibody (ABIN7042970, ABIN7044761 and ABIN7044762) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize BDNF from rat, human, and mouse samples. The antibody is specific for BDNF, it does not crossreact with NGF, or .
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)VLEKVPVSKQLK, corresponding to amino acid residues 166-178 of human BDNF (precursor)
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亚型
- IgG
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anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 20-252) antibody
BDNF 适用: Pig WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 19-248) antibodyBDNF 适用: 人 WB, ICC, ELISA 宿主: 小鼠 Monoclonal 3D9C5 unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 151-247) antibodyBDNF 适用: 人, 大鼠, 小鼠 WB, ICC, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 20-252) antibodyBDNF 适用: Pig WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 1-247) antibodyBDNF 适用: 人 WB, IP, ELISA, IF 宿主: 小鼠 Monoclonal 1B10 unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (Internal Region) antibodyVerified BDNF 适用: 人 ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 206-236), (C-Term) antibodyBDNF 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB14839 unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 129-247) antibodyBDNF 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 206-236) antibodyBDNF 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 129-247) antibodyBDNF 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1:200
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: 1:300
IP: The optimal concentration should be determined by the user
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说明
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Positive Control: PCP-B250
Cited Application: ICC
Negative Control: (ABIN7234876)
Blocking Peptide: (ABIN7234876)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
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别名
- BDNF
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背景
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Synonyms: Brain-derived neurotrophic factor
Description: Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors which includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.BDNF conveys its activity by binding to two classes of receptors, a member of the Trk receptor tyrosine kinase family (TrkB) and the pan-neurotrophin receptor p75NTR. Binding of BDNF to the TrkB receptor triggers ligand-induced dimerization and autophosphorylation of tyrosine residues. This activates various signaling cascades such the MAPK, PI3K and PLCγ pathways that are involved in cell growth, survival and differentiation of neurons in the central and peripheral nervous system.Interestingly, recent evidence suggests that BDNF may influence target cell function via ion channel modulation. Ion channel activity in the target cells can be modulated by a TrkB-mediated mechanism that has not yet been determined. BDNF is able to block both KV1.3 and AMPA-subtype glutamate ion channel currents in sensory neurons, while it can induce activation of the TRPC3 cation channel in neurons and of the NaV1.9 Na+ channel in hippocampal neurons. These newly recognized BDNF actions underlie its "rapid" neuronal functions that include changes in neuronal excitability, plasticity and synaptic transmission.
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基因ID
- 627
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NCBI登录号
- NM_170735
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UniProt
- P23560
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途径
- RTK signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
抗原
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