ASIC3 抗体 (Intracellular)
Quick Overview for ASIC3 抗体 (Intracellular) (ABIN7042916)
抗原
See all ASIC3 (ACCN3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-16, Intracellular
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原理
- A Guinea Pig Polyclonal Antibody to ASIC3 Channel
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特异性
- Intracellular, N-terminus
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交叉反应
- 小鼠, 大鼠
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预测反应
- 15 amino acid residues identical, human - 11,Mouse - 14
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产品特性
- Guinea pig Anti-ASIC3 Antibody is directed against an epitope of rat ASIC3. Guinea pig Anti-ASIC3 Antibody raised in guinea pig can be used in western blot and immunohistochemistry applications. It has been designed to recognize ASIC3 from rat, human, and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-ASIC3 Antibody (ABIN7042917, ABIN7045243 and ABIN7045244) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3
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亚型
- IgG
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anti-Amiloride-Sensitive Cation Channel 3 (ACCN3) (AA 62-320) antibody
ACCN3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Amiloride-Sensitive Cation Channel 3 (ACCN3) (AA 53-102) antibodyACCN3 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, 兔 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Amiloride-Sensitive Cation Channel 3 (ACCN3) (N-Term) antibodyACCN3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Amiloride-Sensitive Cation Channel 3 (ACCN3) (N-Term) antibodyACCN3 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, 兔, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Amiloride-Sensitive Cation Channel 3 (ACCN3) (AA 1-549) antibodyACCN3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Amiloride-Sensitive Cation Channel 3 (ACCN3) (AA 2-16), (Intracellular) antibodyACCN3 适用: 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Amiloride-Sensitive Cation Channel 3 (ACCN3) (Internal Region) antibodyACCN3 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200
Application Dilutions Western blot wb: 1:1000
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说明
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Cited Application: ICC|IHC
Negative Control: (ABIN7234708)
Blocking Peptide: (ABIN7234708)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))
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别名
- ASIC3
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背景
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Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC,ASIC3 is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC1, ASIC2, ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.The functional channel is composed of 4 subunits that can be assembled as homo- or heterotetramers with the other ASIC subunits.A drop in external pH opens the channel resulting in an inward cation current that is quickly inactivated even in the continuous presence of protons in the medium, although a small residual current may persist.Several lines of evidence indicate that ASIC3 may function as a pain sensor. First, it is specifically located in dorsal root ganglia (DRG) neurons and in nociceptors that are involved in pain detection and transmission. In addition, in conditions such as inflammation and cardiac ischemia that include tissue acidosis and thus pain, ASIC3 currents have been detected. Finally, ASIC3 deficient mice display altered sensitivity to high intensity pain produced by heat or acid.Interestingly, while in rats ASIC3 is expressed almost exclusively in DRG neurons, in humans its expression is more widespread suggesting a more extensive role in human nociception.
Alternative names: ASIC3, Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC -
基因ID
- 286920
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NCBI登录号
- NM_004769
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UniProt
- O35240
抗原
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