ADRB1 抗体 (Extracellular)
Quick Overview for ADRB1 抗体 (Extracellular) (ABIN7042902)
抗原
See all ADRB1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 200-214, Extracellular
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原理
- A Rabbit Polyclonal Antibody to β1-Adrenergic Receptor
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特异性
- 2nd extracellular loop
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交叉反应
- 小鼠, 大鼠
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预测反应
- Rat,Mouse,human - 14,15 amino acid residues identical
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产品特性
- Anti-β1-Adrenergic Receptor (extracellular) Antibody (ABIN7042902, ABIN7043924 and ABIN7043925) is a highly specific antibody directed against an extracellular epitope the rat β1-adrenoceptor. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize β1AR from mouse, rat and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RAESDEARR(S)YNDP, corresponding to amino acid residues 200-214 of rat ADRB1
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亚型
- IgG
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anti-Adrenergic, beta-1-, Receptor (ADRB1) (Internal Region) antibody
Verified ADRB1 适用: 人 ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Adrenergic, beta-1-, Receptor (ADRB1) (AA 181-250) antibodyADRB1 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic, beta-1-, Receptor (ADRB1) (Middle Region) antibodyADRB1 适用: 人, 大鼠, 小鼠, 犬, Pig, 兔, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic, beta-1-, Receptor (ADRB1) (AA 401-477) antibodyADRB1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic, beta-1-, Receptor (ADRB1) (AA 150-250) antibodyADRB1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic, beta-1-, Receptor (ADRB1) antibodyADRB1 适用: 人 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Adrenergic, beta-1-, Receptor (ADRB1) (AA 181-250) antibody (FITC)ADRB1 适用: 人, 大鼠, 小鼠 WB, FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal FITC
anti-Adrenergic, beta-1-, Receptor (ADRB1) (AA 180-229) antibodyADRB1 适用: 人, 大鼠, 小鼠, 犬, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7234672)
Blocking Peptide: (ABIN7234672)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- ADRB1 (Adrenergic, beta-1-, Receptor (ADRB1))
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别名
- ADRB1
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背景
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Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR,β-Adrenergic receptors (βAR) belong to the superfamily of G-protein coupled receptors (GPCRs). βAR stimulation by the sympathetic nervous system or circulating catecholamines is involved in peripheral blood circulation, metabolic regulation, muscle contraction, and central neural activities. In the heart, acute βAR stimulation serves as the most powerful means to regulate cardiac output in response to a fight-or-flight situation.There are three βAR subtypes, β1AR, β2AR and β3AR, in cardiac myocytes1. The receptors consist of seven membrane-spanning domains, three intra- and three extracellular loops, an extracellular N-terminal domain, and an intracellular C-terminal tail2.β1AR is the predominant receptor subtype expressed in human cardiomyocytes. β1AR is also expressed in the kidney3. It has been implicated in adipogenesis and memory formation4.β1AR has been widely connected with heart disease. Both the downregulation and overstimulation of β1AR have been connected to myocyte apoptosis. In addition, chronic heart failure is characterized by abnormalities in β1AR signal transduction. It was demonstrated that heart failure is also inducible in animal models by overexpression of components of the β1AR signal transduction cascade. Transgenic mice overexpressing β1AR develop, with aging, heart failure similar to that observed in dilated cardiomyopathy in humans5.
Alternative names: beta1-Adrenergic Receptor, Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR -
基因ID
- 64508
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NCBI登录号
- NM_000684
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UniProt
- P18090
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途径
- cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Brown Fat Cell Differentiation
抗原
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