Adenosine A2a Receptor 抗体 (Extracellular) (PE)
Quick Overview for Adenosine A2a Receptor 抗体 (Extracellular) (PE) (ABIN7042888)
抗原
See all Adenosine A2a Receptor (ADORA2A) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 143-156, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Adenosine A2A Receptor (extracellular) conjugated to the fluorescent dye R-Phycoerythrin (R-PE)
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特异性
- 2nd extracellular loop
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交叉反应
- 小鼠, 大鼠
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交叉反应 （详细）
- It will not recognize A2A Adenosine Receptor from human samples
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预测反应
- Mouse - 13 out of 14 amino acid residues identical, rat - 12 out of 14 amino acid residues identical It will not recognize A2A Adenosine Receptor from human samples
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产品特性
- A Rabbit Polyclonal Antibody to Adenosine A2A Receptor (extracellular) conjugated to the fluorescent dye R-Phycoerythrin (R-PE)
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)SQKDENSTKT(S)GE, corresponding to amino acid residues 143-156 of mouse adenosine A-2A receptor
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亚型
- IgG
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anti-Adenosine A2a Receptor (ADORA2A) (C-Term) antibody
ADORA2A 适用: 人 WB 宿主: 山羊 Polyclonal unconjugated
anti-Adenosine A2a Receptor (ADORA2A) (AA 100-200) antibodyADORA2A 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Adenosine A2a Receptor (ADORA2A) (AA 274-412) antibodyADORA2A 适用: 人 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 6A6G11 unconjugated
anti-Adenosine A2a Receptor (ADORA2A) antibodyADORA2A 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Adenosine A2a Receptor (ADORA2A) (AA 1-412) antibodyADORA2A 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-Adenosine A2a Receptor (ADORA2A) (AA 165-300) antibodyADORA2A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Adenosine A2a Receptor (ADORA2A) (AA 291-412) antibodyADORA2A 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Adenosine A2a Receptor (ADORA2A) (AA 347-381), (C-Term) antibodyADORA2A 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB52103 unconjugated
anti-Adenosine A2a Receptor (ADORA2A) (AA 1-412) antibodyADORA2A 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Adenosine A2a Receptor (ADORA2A) (AA 147-267) antibodyADORA2A 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 4E4 unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: N/A
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说明
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Negative Control: (ABIN7582046)
Blocking Peptide: (ABIN7234630)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 15 μL or 50 μL double distilled water (DDW), depending on the sample size.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Adenosine A2a Receptor (ADORA2A)
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别名
- ADORA2A
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背景
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ADORA2A, A2AAR, Adenosine A2aR,Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart1,2. Adenosine exerts its actions via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular -NH2 terminus, cytoplasmic -COOH terminus, and a third intracellular loop important for binding G proteins. The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs1-3.Adenosine receptors control neurotransmitter release through the facilitatory A2AAR and the inhibitory A1AR.4 A2AAR and A1AR are the major adenosine receptor subtypes expressed in the central nervous system (CNS). A2AAR is mainly expressed in the striatum on GABAergic striatopallidal neurons, while A1AR is widely distributed throughout the CNS5,6.A2AAR was suggested to play a critical role in attenuation of systemic inflammatory responses and prevention of extensive tissue damage.7 It was suggested that extracellular adenosine that accumulates in inflamed and damaged tissue may activate the A2AAR expressed in immune cells leading to termination/inhibition of the immune response.7 It was further suggested that this same mechanism may protect tumors from antitumor T cells through an immunosuppressive signal generated by the activation of A2AAR on T cells by extracellular adenosine produced from hypoxic cancerous tissues8.
Alternative names: ADORA2A, A2AAR, Adenosine A2aR -
基因ID
- 11540
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NCBI登录号
- NM_000675
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UniProt
- Q60613
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途径
- Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints
抗原
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