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CD73 抗体 (Extracellular, N-Term)

The 兔多克隆 anti-CD73 antibody is suitable to detect CD73 in samples from 小鼠. It has been validated for WB, IHC, FACS, IF 和 LCI.

Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 3) and human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 2 and 4): - 1,2. (CD73 抗体 (Extracellular, N-Term))

Expression of CD73 in mouse striatum and hippocampus. (CD73 抗体 (Extracellular, N-Term))

IHC

Cell surface detection of CD73 in live intact human Jurkat T-cell leukemia cells: (black line) Cells. (CD73 抗体 (Extracellular, N-Term))

FACS

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4): - 1,2. (CD73 抗体 (Extracellular, N-Term))

4 images

产品编号 ABIN7042877

发货至: 中国

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Quick Overview for CD73 抗体 (Extracellular, N-Term) (ABIN7042877)

抗原

See all CD73 (NT5E) 抗体

CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

适用

小鼠

宿主

兔

克隆类型

多克隆

标记

This CD73 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Discover our top product NT5E Primary Antibody

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AA 429-433, Extracellular, N-Term

原理

A Rabbit Polyclonal Antibody to CD73

交叉反应

人, 小鼠, 大鼠

预测反应

Human,rat - identical

产品特性

Anti-CD73 (extracellular) Antibody (ABIN7042877, ABIN7044811 and ABIN7044812) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize NT5E from human, rat and mouse samples.

纯化方法

Affinity purified on immobilized antigen.

免疫原

(C)KGSTLKKAFEHSVHR, corresponding to amino acid residues 429-433 of mouse CD73

亚型

IgG
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibody
NT5E 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4G6B10 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 520-550), (C-Term) antibody
NT5E 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB02846 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-500) antibody
NT5E 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody
NT5E 适用: 人 WB, IHC, FACS, IF, ELISA 宿主: 小鼠 Monoclonal NT5E-2545 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody
NT5E 适用: 人 WB, IHC, FACS, IF 宿主: 小鼠 Monoclonal NT5E-2503 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 290-550) antibody
NT5E 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibody
NT5E 适用: 人 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 4G6E3 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 48-527) antibody
NT5E 适用: Cow WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody (PE)
NT5E 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal AD2 PE

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 151-250) antibody
NT5E 适用: 人, 小鼠, 大鼠, Cow, 犬, 绵羊 WB, FACS, ELISA, ICC, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:200
FC: 5 μg per reaction
ICC: The optimal concentration should be determined by the user
IHC: 1:200
IP: The optimal concentration should be determined by the user

说明

Negative Control: (ABIN7234599)
Blocking Peptide: (ABIN7234599)

限制

仅限研究用
状态

Lyophilized

溶解方式

25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

浓度

0.8 mg/mL

缓冲液

PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
抗原

CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

别名

NT5E

背景

Synonyms: Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E
Description: CD73 (ecto-5'-nucleotidase, NT5E) is a glycosylphosphatidylinositol enzyme expressed on the cell surface of various cell types. CD73 is responsible for hydrolyzing extracellular adenosine monophosphate to adenosine and inorganic phosphate, which results in activation of the G-protein coupled receptors, namely A2AR and A2BR, to exert potent immunoregulatory activity.CD73 plays an important role in homeostatic functions in healthy organs and tissues. The protein is involved as an inhibitory immune checkpoint molecule, it promotes immune escape of tumor cells since free adenosine generated by the protein inhibits cellular immune responses1,2.CD73 primarily detected in cancer cells and in immune cells such as CD4+Foxp3+ regulatory T cells, and myeloid-derived suppressor cells that are recruited by the tumor2.Studies show that CD73 expression is significantly up-regulated in numerous malignant tumors such as breast cancer, colorectal cancer, prostate cancer, ovarian cancer, and gallbladder cancer3.

基因ID

23959

NCBI登录号

NM_001204813

UniProt

Q61503

途径

Synaptic Membrane, Ribonucleoside Biosynthetic Process