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CD73 抗体 (Extracellular)

This anti-CD73 antibody is a 兔 多克隆 antibody detecting CD73 in WB, IHC, FACS, IF 和 LCI. Suitable for 小鼠.
产品编号 ABIN7042877
发货至: 中国

Quick Overview for CD73 抗体 (Extracellular) (ABIN7042877)

抗原

See all CD73 (NT5E) 抗体
CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

适用

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多克隆

标记

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This CD73 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)
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    AA 429-433, Extracellular

    原理

    A Rabbit Polyclonal Antibody to CD73

    特异性

    Extracellular, N-terminus

    交叉反应

    人, 小鼠, 大鼠

    预测反应

    Human,rat - identical

    产品特性

    Anti-CD73 (extracellular) Antibody (ABIN7042877, ABIN7044811 and ABIN7044812) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize NT5E from human, rat and mouse samples.

    纯化方法

    Affinity purified on immobilized antigen.

    免疫原

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)KGSTLKKAFEHSVHR, corresponding to amino acid residues 429-433 of mouse CD73

    亚型

    IgG
  • 应用备注

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

    Application Dilutions Western blot wb: 1:200

    说明

    Negative Control: (ABIN7234599)

    Blocking Peptide: (ABIN7234599)

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

    浓度

    1 mg/mL

    缓冲液

    PBS pH 7.4

    储存条件

    4 °C,-20 °C

    储存方法

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • 抗原

    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

    别名

    NT5E

    背景

    Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E,CD73 (ecto-5'-nucleotidase, NT5E) is a glycosylphosphatidylinositol enzyme expressed on the cell surface of various cell types. CD73 is responsible for hydrolyzing extracellular adenosine monophosphate to adenosine and inorganic phosphate, which results in activation of the G-protein coupled receptors, namely A2AR and A2BR, to exert potent immunoregulatory activity.CD73 plays an important role in homeostatic functions in healthy organs and tissues. The protein is involved as an inhibitory immune checkpoint molecule, it promotes immune escape of tumor cells since free adenosine generated by the protein inhibits cellular immune responses1,2.CD73 primarily detected in cancer cells and in immune cells such as CD4+Foxp3+ regulatory T cells, and myeloid-derived suppressor cells that are recruited by the tumor2.Studies show that CD73 expression is significantly up-regulated in numerous malignant tumors such as breast cancer, colorectal cancer, prostate cancer, ovarian cancer, and gallbladder cancer3.

    Alternative names: CD73, Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E

    基因ID

    23959

    NCBI登录号

    NM_001204813

    UniProt

    Q61503

    途径

    Synaptic Membrane, Ribonucleoside Biosynthetic Process
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