EPH Receptor B6 抗体 (Internal Region)
Quick Overview for EPH Receptor B6 抗体 (Internal Region) (ABIN7013872)
抗原
See all EPH Receptor B6 (EPHB6) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
-
-
抗原表位
- Internal Region
-
原理
- EPHB6
-
序列
- EVIAHGKHTT SSD
-
交叉反应
- 犬, 人, 小鼠, 大鼠
-
纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
-
-
-
应用备注
-
Immunohistochemistry: Paraffin embedded Human Small Intestine and Brain (Cerebellum). Recommended concentration: 3.75 μg/mL.
Western Blot: Preliminary testing showed a band at approx 80 kDa in U251 cell lysate and approx.75 kDa in Human Pancreas lysate after 1-2 μg/mL antibody staining (calculated MW of 79.8 kDa according to NP_001267723.2). Primary incubation 1 hour at room tem
Peptide ELISA: antibody detection limit dilution 1:32000.
-
说明
-
Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended c
-
限制
- 仅限研究用
-
-
-
缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Minimize freezing and thawing.
-
储存条件
- -20 °C
-
储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
-
- EPH Receptor B6 (EPHB6)
-
别名
- EPHB6
-
背景
- EPHB6, EPH receptor B6, HEP, MGC129910, MGC129911, ephrin receptor EphB6
-
基因ID
- 2051, 13848, 312275
-
途径
- RTK signaling, Hormone Transport
抗原
-