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beta Actin 抗体

This anti-beta Actin antibody is a 小鼠 单克隆 antibody detecting beta Actin in WB 和 IHC (p). Suitable for 人, 小鼠, 大鼠, 小鸡, 兔, 犬, 猴, Hamster 和 Insect.
产品编号 ABIN7250848
发货至: 中国

Quick Overview for beta Actin 抗体 (ABIN7250848)

抗原

See all beta Actin (ACTB) 抗体
beta Actin (ACTB) (Actin, beta (ACTB))

适用

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人, 小鼠, 大鼠, 小鸡, 兔, 犬, 猴, Hamster, Insect

宿主

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小鼠

克隆类型

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单克隆

标记

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This beta Actin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

2M3
  • 产品特性

    Control Antibody

    纯化方法

    Protein A purification

    免疫原

    Synthetic Peptide

    亚型

    IgG
  • 应用备注

    WB 1:2000-1:5000, IHC 1:50-1:200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS with 0.02 % sodium azide and 50 % glycerol pH 7.4.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20°C. Avoid freeze / thaw cycles.
  • 抗原

    beta Actin (ACTB) (Actin, beta (ACTB))

    别名

    beta actin

    背景

    Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins. 

    分子量

    Observed_MW: 43 kDa

    Calculated_MW: 42 kDa

    UniProt

    P60709

    途径

    Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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