AKT1 抗体 (AA 122-443)
Quick Overview for AKT1 抗体 (AA 122-443) (ABIN7439390)
抗原
See all AKT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 122-443
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原理
- Polyclonal Antibody to Protein Kinase B Alpha (PKBa)
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特异性
- The antibody is a rabbit polyclonal antibody raised against PKBa. It has been selected for its ability to recognize PKBa in immunohistochemical staining and western blotting.
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交叉反应
- 小鸡, Cow, 犬, 小鼠, Pig, 大鼠
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纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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免疫原
- Recombinant Protein Kinase B Alpha (PKBa) corresdonding to Ser122~Thr443 with N-terminal His Tag
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亚型
- IgG
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应用备注
- Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.
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说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
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储存液
- ProClin, Sodium azide
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注意事项
- This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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有效期
- 24 months
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- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
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别名
- PKBa
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背景
- AKT1, AKT, PKB-A, Proto-oncogene c-Akt, RAC-Alpha Serine/Threonine-Protein Kinase, V-akt Murine Thymoma Viral Oncogene Homolog 1
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UniProt
- P31749
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途径
- PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect
抗原
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