PKC alpha 抗体 (AA 339-597)
Quick Overview for PKC alpha 抗体 (AA 339-597) (ABIN7434338)
抗原
See all PKC alpha (PKCa) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 339-597
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原理
- Polyclonal Antibody to Protein Kinase C Alpha (PKCa)
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特异性
- The antibody is a rabbit polyclonal antibody raised against PKCa. It has been selected for its ability to recognize PKCa in immunohistochemical staining and western blotting.
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交叉反应
- 人, 大鼠
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纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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免疫原
- Recombinant Protein Kinase C Alpha (PKCa) corresdonding to Phe339~Phe597 (Accession # P20444)
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亚型
- IgG
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应用备注
- Western blotting: 0.5-2 μg/mL,1:500-2000,Immunohistochemistry: 5-20 μg/mL,1:50-200,Immunocytochemistry: 5-20 μg/mL,1:50-200,Optimal working dilutions must be determined by end user.
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说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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有效期
- 24 months
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- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
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别名
- PKCa
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背景
- PRKCA, PKC-A, AAG6, PKC-Alpha
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UniProt
- P20444
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途径
- WNT signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
抗原
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