CD25 抗体 (AA 22-245)
Quick Overview for CD25 抗体 (AA 22-245) (ABIN7443365)
抗原
See all CD25 (IL2RA) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 22-245
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原理
- Polyclonal Antibody to Interleukin 2 Receptor Alpha (IL2Ra)
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特异性
- The antibody is a rabbit polyclonal antibody raised against IL2Ra. It has been selected for its ability to recognize IL2Ra in immunohistochemical staining and western blotting.
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纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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免疫原
- Recombinant Interleukin 2 Receptor Alpha (IL2Ra) corresdonding to Gly22~Gln245 (Accession # O02733)
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亚型
- IgG
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应用备注
- Western blotting: 1:50-400,Immunocytochemistry in formalin fixed cells: 1:50-500,Immunohistochemistry in formalin fixed frozen section: 1:50-500,Immunohistochemistry in paraffin section: 1:10-100,Enzyme-linked Immunosorbent Assay: 1:100-5000,Optimal working dilutions must be determined by end user.
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说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 500 μg/mL
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缓冲液
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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有效期
- 24 months
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- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
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别名
- IL2Ra
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背景
- CD25, IL2R-a, IL2-RA, IDDM10, IL2R, TCGFR, p55, TAC antigen
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UniProt
- O02733
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途径
- JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
抗原
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