ube3a 抗体 (AA 158-407)
Quick Overview for ube3a 抗体 (AA 158-407) (ABIN7433114)
抗原
See all ube3a 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 158-407
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原理
- Polyclonal Antibody to Ubiquitin Protein Ligase E3A (UBE3A)
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特异性
- The antibody is a rabbit polyclonal antibody raised against UBE3A. It has been selected for its ability to recognize UBE3A in immunohistochemical staining and western blotting.
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纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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免疫原
- Recombinant Ubiquitin Protein Ligase E3A (UBE3A) corresdonding to Ser158~Leu407 with N-terminal His Tag
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亚型
- IgG
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应用备注
- Western blotting: 1-5 μg/mLImmunocytochemistry in formalin fixed cells: 5-20 μg/mLImmunohistochemistry in formalin fixed frozen section: 5-20 μg/mLImmunohistochemistry in paraffin section: 5-20 μg/mLEnzyme-linked Immunosorbent Assay: 0.05-2 μg/mLOptimal working dilutions must be determined by end user.
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说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 500 μg/mL
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缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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有效期
- 24 months
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- ube3a (Ubiquitin Protein Ligase E3A (ube3a))
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别名
- UBE3A
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背景
- ANCR, AS, E6-AP, EPVE6AP, HPVE6A, Papilloma Virus E6-Associated,Angelman Syndrome, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54
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UniProt
- Q05086
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway
抗原
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