SIRT1 抗体 (AA 238-482)
Quick Overview for SIRT1 抗体 (AA 238-482) (ABIN7427144)
抗原
See all SIRT1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 238-482
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原理
- Monoclonal Antibody to Sirtuin 1 (SIRT1)
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特异性
- The antibody is a mouse monoclonal antibody raised against SIRT1. It has been selected for its ability to recognize SIRT1 in immunohistochemical staining and western blotting.
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纯化方法
- Protein A + Protein G affinity chromatography
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免疫原
- Recombinant Sirtuin 1 (SIRT1) corresdonding to Lys238~Ala482 (Accession # 149043900)
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亚型
- IgG
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应用备注
- Western blotting: 0.5-2 μg/mL,1:500-2000Immunohistochemistry: 5-20 μg/mL,1:50-200Immunocytochemistry: 5-20 μg/mL,1:50-200Optimal working dilutions must be determined by end user.
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说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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有效期
- 24 months
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- SIRT1 (Sirtuin 1 (SIRT1))
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别名
- SIRT1
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背景
- SIR2L1, Silent Mating Type Information Regulation 2 Homolog 1, NAD-Dependent Deacetylase Sirtuin-1, SIR2-like protein 1, SirtT1 75 kDa fragment
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UniProt
- A0A8I6A306
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途径
- MAPK Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling
抗原
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