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beta Actin 抗体 (AA 1-375)

This anti-beta Actin antibody is a 小鼠 单克隆 antibody detecting beta Actin in WB, IHC, ICC 和 IP. Suitable for 人, 小鼠, 大鼠, 犬, Pig 和 Cow.
产品编号 ABIN7428920
发货至: 中国

Quick Overview for beta Actin 抗体 (AA 1-375) (ABIN7428920)

抗原

See all beta Actin (ACTB) 抗体
beta Actin (ACTB) (Actin, beta (ACTB))

适用

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人, 小鼠, 大鼠, 犬, Pig, Cow

宿主

  • 117
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  • 5
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小鼠

克隆类型

  • 112
  • 111
单克隆

标记

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This beta Actin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

克隆位点

C5
  • 抗原表位

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    AA 1-375

    原理

    Monoclonal Antibody to Beta Actin (ACTB)

    特异性

    The antibody is a mouse monoclonal antibody raised against ACTB. It has been selected for its ability to recognize ACTB in immunohistochemical staining and western blotting.

    交叉反应

    纯化方法

    Protein A + Protein G affinity chromatography

    免疫原

    Recombinant Beta Actin (ACTB) corresdonding to Met1~Phe375 with N-terminal His Tag

    亚型

    IgG2b kappa
  • 应用备注

    Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    说明

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    有效期

    24 months
  • 抗原

    beta Actin (ACTB) (Actin, beta (ACTB))

    别名

    ACTB

    背景

    Actin Beta, ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed

    途径

    Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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