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EGF 抗体 (AA 609-751)

This anti-EGF antibody is a 小鼠 单克隆 antibody detecting EGF in WB, IHC, IP 和 ICC. Suitable for 人.
产品编号 ABIN7428500
发货至: 中国

Quick Overview for EGF 抗体 (AA 609-751) (ABIN7428500)

抗原

See all EGF 抗体
EGF (Epidermal Growth Factor (EGF))

适用

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宿主

  • 211
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  • 1
小鼠

克隆类型

  • 214
  • 45
单克隆

标记

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This EGF antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

克隆位点

D2
  • 抗原表位

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    AA 609-751

    原理

    Monoclonal Antibody to Epidermal Growth Factor (EGF)

    特异性

    The antibody is a mouse monoclonal antibody raised against EGF. It has been selected for its ability to recognize EGF in immunohistochemical staining and western blotting.

    交叉反应

    Pig

    纯化方法

    Protein A + Protein G affinity chromatography

    免疫原

    Recombinant Epidermal Growth Factor (EGF) corresdonding to Ala609~Gly751 with N-terminal His Tag

    亚型

    IgG2b kappa
  • 应用备注

    Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    说明

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    有效期

    24 months
  • 抗原

    EGF (Epidermal Growth Factor (EGF))

    别名

    Epidermal Growth Factor

    背景

    URG, Beta-Urogastrone, Pro-epidermal growth factor

    UniProt

    P01133

    途径

    NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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