Betatrophin antibody is human, mouse and rat reactive. At least three isoforms of Betatrophin are known to exist, this antibody will detect the two largest isoforms.
纯化方法
Betatrophin antibody is affinity chromatography purified via peptide column.
免疫原
Betatrophin antibody was raised against a 16 amino acid peptide near the amino terminus of human Betatrophin. The immunogen is located within amino acids 40 - 90 of Betatrophin.
C19ORF80
适用: 小鼠
IF (cc), IF (p)
宿主: 小鼠
Monoclonal
22G2
Alexa Fluor 488
应用备注
Betatrophin antibody can be used for detection of Betatrophin by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in rat samples, Immunohistochemistry in mouse and rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Betatrophin antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
Betatrophin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
抗原
C19ORF80
(Chromosome 19 Open Reading Frame 80 (C19ORF80))
The Betatrophin protein, also known as the hepatocellular carcinoma-associated protein TD26, is an adipocyte- and liver-enriched insulin targeted gene that is thought to play a role in lipid metabolism be regulating serum triglyceride levels (1,2). Betatrophin is closely related to the angiopoietin-like (ANGPTL) protein family. Betatrophin mRNA increases in the cold environment and after treatment with a high-fat diet, while its mRNA levels decrease in response to fasting, suggesting that it may play a role in mediating thermogenesis and energy homeostasis (3).