NKX2-2 antibody is predicted to not cross-react with other NK2 homeobox family members.
纯化方法
NKX2-2 Antibody is affinity chromatography purified via peptide column.
免疫原
NKX2-2 antibody was raised against a 19 amino acid synthetic peptide near the center of human NKX2-2. The immunogen is located within amino acids 90 - 140 of NKX2-2.
NKX2-2 antibody can be used for detection of NKX2-2 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
NKX2-2 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
NKX2-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原
Nkx2-2
(NK2 Homeobox 2 (Nkx2-2))
别名
NKX2-2
背景
NKX2-2 Antibody: NKX2-2 (NK2 homeobox 2) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-2 is crucial in the development of pancreatic islet cell differentiation and activates the ghrelin-promoter in these cells. NKX2-2 is expressed at the onset of pancreatic epithelium formation and becomes restricted to mature α,-, β,- and PP cells, and in NKX2-2-null mice embryos, all β,-cells and most a-cells are replaced by ghrelin-positive cells. NKX2-2 is also thought to be involved in ventral neuronal patterning and floor plate development and commissural axon guidance.