beta Actin 抗体 (N-Term)
Quick Overview for beta Actin 抗体 (N-Term) (ABIN6990626)
抗原
See all beta Actin (ACTB) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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纯化方法
- beta-Actin Antibody is affinity chromatography purified via peptide column.
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免疫原
- b-actin antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human b-actin.
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亚型
- IgG
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应用备注
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b-actin antibody can be used for the detection of b-actin by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 1 μ,g/mL. For immunofluorescence start at 2 μ,g/mL.
Antibody validated: Western Blot in human and mouse samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- beta-Actin Antibody is supplied in PBS containing 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,4 °C
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储存方法
- beta-Actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- beta Actin (ACTB) (Actin, beta (ACTB))
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别名
- beta-Actin
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背景
- Beta-Actin Antibody: Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
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基因ID
- 60
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UniProt
- P60709
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途径
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
抗原
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