Immunohistochemistry (IHC), Supershift Assay (SSA), Western Blotting (WB)
产品特性
RAR-b (HAP, NR1B2) is a member of the thyroid-steroid hormone receptor superfamily of nuclear transcriptional regulators. RAR-b localizes to the cytoplasm and to subnuclear compartments. RAR-b binds retinoic acid, the biologically active form of vitamin A which mediates cellular signaling in embryonic morphogenesis, cell growth and differentiation. It is thought that RAR-b limits growth of many cell types by regulating gene expression. RAR-beta antibody (mAb) (Clone 8B-10B2) was raised in a Mouse host. It has been validated for use in Immunohistochemistry, Supershift and Western blot, it has been shown to react with Human samples.
纯化方法
Protein G Chromatography
免疫原
This RAR-beta antibody was raised against amino acids 413-448 of human RAR-beta.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.