L-Selectin 抗体 (redFluor™ 710)
Quick Overview for L-Selectin 抗体 (redFluor™ 710) (ABIN7278600)
抗原
See all L-Selectin (SELL) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
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亚型
- IgG2a kappa
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应用备注
- This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. redFluor™ 710 dye is excited by the red (633-647 nm) laser and has a peak emission of 710 nm. The recommended band pass filter for this dye is 710/50. redFluor™ 710 can be used as an alternative for Alexa Fluor® 700. Confirm that your cytometer is configured to detect this fluorochrome.
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说明
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0.2 mg/mL
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限制
- 仅限研究用
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缓冲液
- 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- 2-8°C protected from light
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有效期
- 12 months
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- L-Selectin (SELL) (Selectin L (SELL))
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别名
- CD62L (L-Selectin)
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背景
- The MEL-14 antibody is specific for mouse CD62L, also known as L-Selectin, a cell adhesion molecule which facilitates lymphocyte ""rolling"" on activated vascular endothelium and homing to high endothelial venules (HEV) as immune cells transmigrate from blood into peripheral tissues. L-Selectin is a member of a family of Selectin molecules which act together with the integrin family of adhesion molecules to mediate leukocyte-endothelial interactions. L-Selectin is characteristically expressed by neutrophils, and is also found on B cells, monocytes, granulocytes, and at varying levels on naive, effector and memory T cells. It is rapidly shed upon cell activation, releasing into the circulation a soluble form whose biological role is of particular interest in cancer biology research.The MEL -14 antibody may be used as a phenotypic marker for CD62L expression on a variety of immune cell types. Please note that CD62L (L-Selectin) itself is also referred to as MEL-14 in the literature.
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基因ID
- 20343
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UniProt
- P18337
抗原
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