Recombinant RFP 抗体 (AZDye 568)
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- 抗原 See all RFP 抗体
- RFP (Red Fluorescent Protein (RFP))
- 抗体类型
- Recombinant Antibody
- 片段
- single-domain Antibody (sdAb)
- 适用
- Discosoma
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宿主
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Alpaca
- Expression System
- E.coli
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克隆类型
- 单克隆
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标记
- This RFP antibody is conjugated to AZDye 568
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- 原理
- Camelid sdAb anti-RFP conjugated with AZDye 568 (Alexa Fluor 568 equivalent), Clones 2B12/2A1
- 特异性
- Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.
- 交叉反应 (详细)
- Does not cross-react with GFP or mTagBFP derivatives.
- 产品特性
- A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
- 纯化方法
- Produced in: E.coli
- 克隆位点
- 2B12-2A1
- Labeling Ratio
- A blend of 2 different sdAbs that bind at different epitopes of the same target protein, each sdAb bears 2 site-specifically coupled fluorophores.
- Top Product
- Discover our top product RFP Primary Antibody
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- 应用备注
- Recommended dilution 1:500
- 说明
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A blend of 2 different sdAbs that bind at different epitopes of the same target protein, two fluorophore molecules are site-specifically coupled to each individual sdAb molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this product can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render this products excellent tools for all microscopy techniques.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- 5 μM
- 缓冲液
- 1.25 μM of each fluorescently labeled sdAb clone in buffered saline, 50 % glycerol, 0.09 % sodium azide. 5 μM total fluorophore concentration
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light!
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
- 有效期
- 12 months
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- 抗原
- RFP (Red Fluorescent Protein (RFP))
- 别名
- RFP (RFP 产品)
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