KRT77, KRT76 抗体
Quick Overview for KRT77, KRT76 抗体 (ABIN6941403)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
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纯化方法
- Purified by Protein A/G
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免疫原
- Human epidermal keratin
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亚型
- IgG1 kappa
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anti-KRT77, KRT76 antibody
适用: 人, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal KRTL-1077-KRTH-1076 unconjugated
anti-KRT77, KRT76 antibody适用: 人 IHC, StM 宿主: 兔 Monoclonal KRTH-1576R-KRTL-1577R unconjugated Recombinant Antibody
anti-KRT77, KRT76 antibody适用: 人 FACS, IF, IHC, StM, WB 宿主: 兔 Polyclonal unconjugated
anti-KRT77, KRT76 antibody (PE)适用: 人, 大鼠, 小鸡, Cow, 犬, 猴, 小鼠, 兔 FACS, IF, T 宿主: 小鼠 Monoclonal SPM115-SPM116 PE
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应用备注
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Positive Control: Skin, Adeno- or Squamous carcinomas.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL for 2 hours at RT), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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