KRT77, KRT76 抗体
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- 抗原
- KRT77, KRT76
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
- 交叉反应 （详细）
- Human. Monkey. Cow. Dog. Rabbit. Mouse. Rat. Chicken.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Human epidermal keratin
- 克隆位点
- SPM115-SPM116
- 亚型
- IgG1 kappa
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anti-KRT77, KRT76 antibody
适用: 人, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal KRTL-1077-KRTH-1076 unconjugated
anti-KRT77, KRT76 antibody适用: 人 FACS, IF, IHC, StM, WB 宿主: 兔 Polyclonal unconjugated
anti-KRT77, KRT76 antibody适用: 人 IHC, StM 宿主: 兔 Monoclonal KRTH-1576R-KRTL-1577R unconjugated Recombinant Antibody
anti-KRT77, KRT76 antibody (PE)适用: 人, 大鼠, 猴, 小鼠, 兔, 小鸡, Cow, 犬 FACS, IF, T 宿主: 小鼠 Monoclonal SPM115-SPM116 PE
anti-KRT77, KRT76 antibody适用: 人, 大鼠, 猴, 小鼠, 兔, 小鸡, Cow, 犬 FACS, IF, IHC, StM, WB 宿主: 小鼠 Monoclonal SPM115-SPM116 unconjugated
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- 应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL for 2 hours at RT), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Skin, Adeno- or Squamous carcinomas.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- KRT77, KRT76
- 别名
- KRT77 & KRT76
- 背景
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K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39,Cytokeratin, pan (Epithelial Marker)
Cellular localisation: Cytoplasmic - 分子量
- 67kDa (CK1), 64kDa (CK3), 59kDa (CK4), 58kDa (CK5), 56kDa (CK6), 52kDa (CK8), 56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19)
- 基因ID
- 374454, 51350, 334989
- UniProt
- Q7Z794, Q01546
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