Keratin 5/8 抗体
Quick Overview for Keratin 5/8 抗体 (ABIN6941336)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
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无交叉反应
- 小鸡, 非洲爪蟾
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纯化方法
- Purified by Protein A/G
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免疫原
- Cytoskeletal preparation from HeLa cells
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亚型
- IgG1 kappa
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anti-Keratin 5/8 (KRT5/8) antibody
KRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, 兔 WB, FACS, IHC, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal RCK102 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, Cow, 绵羊 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal C-50 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, 兔 WB, FACS, IHC, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal RCK102 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, Cow, 绵羊, 雪貂 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal SPM268 unconjugated
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应用备注
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Positive Control: HepG2 or A431 cells. Skin or Squamous cell carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 0.5 mL at 200 µg/mL of antibody Purified from Bioreactor Concentrate by Protein A/G chromatography. Supplied in 10 mM PBS, pH 7.4 with 0.05 % BSA and 0.05 % sodium azide. Contact us if you require this antibody in a different format.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2 to 8°C. Antibody is stable for 24 months.
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有效期
- 24 months
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