Recombinant CD27 抗体
Quick Overview for Recombinant CD27 抗体 (ABIN6941125)
抗原
See all CD27 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes a protein of a disulfide-linked 120 kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFkB and SAPK/JNK and induces apoptosis.
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant human CD22 protein (exact sequence is proprietary)
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亚型
- IgG1 kappa
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anti-CD27 Molecule (CD27) (AA 1-260) antibody
CD27 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 1E2-A3 unconjugated
anti-CD27 Molecule (CD27) antibodyCD27 适用: 人 FACS, ELISA, IHC, IF, StM, Coat 宿主: 小鼠 Monoclonal LPFS2-1611 unconjugated
anti-CD27 Molecule (CD27) antibodyCD27 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD27 Molecule (CD27) (AA 20-191) antibodyCD27 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD27 Molecule (CD27) (AA 20-144) antibodyCD27 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 3B6D3 unconjugated
anti-CD27 Molecule (CD27) (AA 196-225) antibodyCD27 适用: 人 FACS, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD27 Molecule (CD27) (AA 229-312) antibodyCD27 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD27 Molecule (CD27) antibodyCD27 适用: 人 FACS 宿主: 小鼠 Monoclonal LT27 unconjugated
anti-CD27 Molecule (CD27) (AA 20-191) antibodyCD27 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM57 unconjugated Recombinant Antibody
anti-CD27 Molecule (CD27) (AA 28-170) antibodyCD27 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-027M unconjugated
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应用备注
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Positive Control: Ramos cells. Human peripheral blood lymphocytes. Human tonsil and lymph node.
Known Application: Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- CD27 (CD27 Molecule (CD27))
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别名
- CD27
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分子量
- 120kDa
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基因ID
- 939
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UniProt
- P26842
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途径
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
抗原
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