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CD27 抗体

CD27 适用: 人 FACS, ELISA, IHC, IF, StM, Coat 宿主: 小鼠 Monoclonal LPFS2-1611 unconjugated
产品编号 ABIN6941124
发货至: 中国
  • 抗原 See all CD27 抗体
    CD27 (CD27 Molecule (CD27))
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    小鼠
    克隆类型
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    单克隆
    标记
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    This CD27 antibody is un-conjugated
    应用范围
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    Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM), Coating (Coat)
    特异性
    Recognizes a protein of a disulfide-linked 120 kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFB and SAPK/JNK and induces apoptosis.
    纯化方法
    Purified by Protein A/G
    免疫原
    Recombinant full-length human CD27 protein
    克隆位点
    LPFS2-1611
    亚型
    IgG1 kappa
  • 应用备注

    Positive Control: Ramos cells. Human peripheral blood lymphocytes. Ramos and lymph node cell lysates. Human tonsil and stomach tissues.

    Known Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    有效期
    24 months
  • 抗原
    CD27 (CD27 Molecule (CD27))
    别名
    CD27 (CD27 产品)
    别名
    TNFRSF7 antibody, S152 antibody, Tnfrsf7 antibody, Tp55 antibody, S152. LPFS2 antibody, T14 antibody, CD27 molecule antibody, CD27 antigen antibody, CD27 antibody, Cd27 antibody
    分子量
    120kDa
    基因ID
    939
    UniProt
    P26842
    途径
    Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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