Calpastatin 抗体
Quick Overview for Calpastatin 抗体 (ABIN6940960)
抗原
See all Calpastatin (CAST) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant full-length human CAST protein
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亚型
- IgG1 kappa
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anti-Calpastatin (CAST) (AA 1-667) antibody
CAST 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 582-669) antibodyCAST 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C5 unconjugated
anti-Calpastatin (CAST) (AA 275-310) antibodyCAST 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 1-350) antibodyCAST 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 431-530) antibodyCAST 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 275-700) antibodyCAST 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 1-686) antibodyCAST 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 1-667) antibodyCAST 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Calpastatin (CAST) antibody (FITC)CAST 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal FITC
anti-Calpastatin (CAST) (AA 550-650) antibodyCAST 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Positive Control: HeLa cells. Esophagus or Small Intestine.
Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Calpastatin (CAST)
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别名
- CAST
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背景
- Calpastatin is an intracellular calcium-dependent protease that cleaves cytoskeletal and sub-membranous proteins. Calpains are non-lysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+- dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpastatin, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpastatin co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.
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分子量
- 126kDa
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基因ID
- 831
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UniProt
- P20810
抗原
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