VEGFA 抗体
Quick Overview for VEGFA 抗体 (ABIN6940869)
抗原
See all VEGFA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This MAb recognizes proteins of 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet-derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.
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纯化方法
- Purified by Protein A/G
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免疫原
- Human VEGF189 recombinant protein
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亚型
- IgG1 kappa
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anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 28-147) antibody
VEGFA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) antibodyVEGFA 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-214) antibodyVEGFA 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-126) antibodyVEGFA 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 207-371) antibodyVEGFA 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 6G5B5 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 33-48), (N-Term) antibodyVEGFA 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-191) antibodyVEGFA 适用: 人, 大鼠, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-130) antibodyVEGFA 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3F7 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA 适用: 人 WB, ELISA, Neut 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Positive Control: Tumor cells in astrocytomas, breast or ovarian carcinomas.
Known Application: ELISA (For coating, order Ab without BSA),Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA buffer, pH 8.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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别名
- VEGFA
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分子量
- 19-22kDa (reducing) and 38kDa-44kDa (non-reducing)
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基因ID
- 7422
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UniProt
- P15692
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途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
抗原
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