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TGFA 抗体 (AA 34-43)

The 小鼠 单克隆 anti-TGFA antibody is suitable to detect TGFA in samples from 人, 兔 和 斑马鱼. It has been validated for IHC, IF, FACS 和 StM.
产品编号 ABIN6940719
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for TGFA 抗体 (AA 34-43) (ABIN6940719)

抗原

See all TGFA 抗体
TGFA (Transforming Growth Factor, alpha (TGFA))

适用

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人, 兔, 斑马鱼

宿主

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小鼠

克隆类型

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单克隆

标记

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This TGFA antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

克隆位点

TG86
  • 抗原表位

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    AA 34-43

    特异性

    This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

    纯化方法

    Purified by Protein A/G

    免疫原

    A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGFα.

    亚型

    IgG1 kappa
  • 应用备注

    Positive Control: Jurkat or Ramos cells. Testicular Carcinoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    TGFA (Transforming Growth Factor, alpha (TGFA))

    别名

    TGFA

    分子量

    ~6kDa

    基因ID

    7039

    UniProt

    P01135

    途径

    NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
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