TGFA 抗体 (AA 34-43)
Quick Overview for TGFA 抗体 (AA 34-43) (ABIN6940717)
抗原
See all TGFA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 34-43
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特异性
- This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
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免疫原
- A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGFα.
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亚型
- IgM kappa
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应用备注
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Positive Control: Jurkat or Ramos cells. Testicular Carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- TGFA (Transforming Growth Factor, alpha (TGFA))
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别名
- TGFA
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分子量
- ~6kDa
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基因ID
- 7039
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UniProt
- P01135
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途径
- NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
抗原
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