RPA2 抗体
Quick Overview for RPA2 抗体 (ABIN6940535)
抗原
See all RPA2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes a protein of 34 kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70 kDa, 32-34 kDa, and 11-14 kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant human full-length Replication protein A2 (RPA2) protein
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亚型
- IgG2b kappa
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应用备注
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Positive Control: MCF-7, HeLa or HepG2 cells. Human tonsil (IHC).
Known Application: Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- RPA2 (Replication Protein A2, 32kDa (RPA2))
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别名
- RPA2
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分子量
- 32-34kDa
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基因ID
- 6118
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UniProt
- P15927
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途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
抗原
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