POU2AF1 抗体 (AA 148-255)
Quick Overview for POU2AF1 抗体 (AA 148-255) (ABIN6940353)
抗原
See all POU2AF1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 148-255
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein (exact sequence is proprietary)
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亚型
- IgG2b kappa
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应用备注
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Positive Control: Raji, Ramos cells. Spleen or Tonsil.
Known Application: Flow Cytometry (1-2 μg/mL),Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))
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别名
- POU2AF1
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背景
- BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells.Expression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia.B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
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分子量
- 35kDa
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基因ID
- 5450
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UniProt
- Q16633
抗原
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