Cytochrome C 抗体 (AA 1-80)
Quick Overview for Cytochrome C 抗体 (AA 1-80) (ABIN6940319)
抗原
See all Cytochrome C (CYCS) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-80
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特异性
- It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
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纯化方法
- Purified by Protein A/G
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免疫原
- Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c
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亚型
- IgG2b kappa
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anti-Cytochrome C, Somatic (CYCS) (AA 51-105) antibody
CYCS 适用: 人, 大鼠, 小鼠, 兔, 小鸡 WB, FACS, ELISA, IHC (p), ICC, IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 1-105) antibodyCYCS 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 1-105) antibodyCYCS 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 5-100) antibodyCYCS 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 13-99) antibodyCYCS 适用: 人, 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 5-100) antibodyCYCS 适用: 人 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 1-105) antibodyCYCS 适用: 人, 小鼠 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 5E6B11 unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 2-105) antibodyCYCS 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 2-105) antibodyCYCS 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 91-105), (C-Term) antibodyCYCS 适用: 人, 大鼠, 小鼠 WB, IHC, FACS, IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
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别名
- CYCS
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分子量
- 15kDa
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基因ID
- 54205
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UniProt
- P99999
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途径
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
抗原
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