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Cytokeratin 18 抗体

This anti-Cytokeratin 18 antibody (ABIN6939932) is a Mouse Monoclonal antibody detecting Cytokeratin 18 in WB, IHC, IF, FACS, StM. Suitable for Human.
产品编号 ABIN6939932
发货至: 中国

Quick Overview for Cytokeratin 18 抗体 (ABIN6939932)

抗原

See all Cytokeratin 18 (KRT18) 抗体
Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Cytokeratin 18 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

克隆位点

KRT18-834
  • 特异性

    This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

    无交叉反应

    牛, 犬, Hamster, 小鼠, 猪, 大鼠, 绵羊

    纯化方法

    Purified by Protein A/G

    免疫原

    Recombinant full-length human KRT18 protein

    亚型

    IgG1 kappa
  • 应用备注

    Positive Control: HCT116, MCF-7, HeLa, A431 cells. Stomach, Intestine or Breast Carcinoma.

    Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

    别名

    KRT18

    分子量

    45kDa

    基因ID

    3875

    UniProt

    P05783

    途径

    Apoptosis, Caspase Cascade in Apoptosis
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