14-3-3 sigma/SFN 抗体
Quick Overview for 14-3-3 sigma/SFN 抗体 (ABIN6939540)
抗原
See all 14-3-3 sigma/SFN (SFN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant human full-length SFN protein
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亚型
- IgG1 kappa
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anti-Stratifin (SFN) antibody
SFN 适用: 人 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Stratifin (SFN) (AA 1-248) antibodySFN 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 3C3 unconjugated
anti-Stratifin (SFN) (Internal Region) antibodyVerified SFN 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Stratifin (SFN) (C-Term) antibodySFN 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Stratifin (SFN) (AA 1-248) antibodySFN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Stratifin (SFN) (AA 140-156) antibodySFN 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Stratifin (SFN) (AA 1-249) antibodySFN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3C3 unconjugated
anti-Stratifin (SFN) (AA 1-248) antibodySFN 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Stratifin (SFN) (AA 140-156), (Middle Region) antibodySFN 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Stratifin (SFN) (AA 1-248) antibodySFN 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Positive Control: PC-3 cells, Skin and tissues enriched in stratified squamous keratinizing epithelium. HaCaT, hTCEpi, A-431 cell lines.
Known Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
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别名
- SFN
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背景
- SFN (stratifin) is a p53-induced tumor suppressor gene which is activated in response to DNA damage, causing cell cycle arrest at G2 phase by blocking cdc2-cyclin B1 complex from entering the nucleus. It is inactivated in breast, lung, prostate, liver and gastric cancer. It is associated with poor prognosis when its down-regulation is observed in epithelial ovarian cancer. SFN expression could contribute to cancer cell proliferation and the development and/or progression of human gastrointestinal cancer.
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分子量
- 28kDa
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基因ID
- 2810
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UniProt
- P31947
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途径
- p53 Pathway, Myometrial Relaxation and Contraction
抗原
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