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14-3-3 sigma/SFN 抗体 (Internal Region)

Verified SFN 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN185539
发货至: 中国
  • 抗原 See all 14-3-3 sigma/SFN (SFN) 抗体
    14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
    抗原表位
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    Internal Region
    适用
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    • 34
    • 27
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    人, 小鼠, 大鼠
    宿主
    • 93
    • 9
    • 1
    • 1
    山羊
    克隆类型
    • 87
    • 17
    多克隆
    标记
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    This 14-3-3 sigma/SFN antibody is un-conjugated
    应用范围
    • 85
    • 43
    • 37
    • 23
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    • 9
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    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    原理
    14-3-3 sigma / Stratifin
    序列
    DLHTLSEDSY KDST
    交叉反应
    犬, 人, 小鼠, Pig, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-DLHTLSEDSYKDST, from the internal region of the protein sequence according to NP_006133.1.
    亚型
    IgG
    Top Product
    Discover our top product SFN Primary Antibody
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer. Recommended concentration, 2-4 μg/mL.

    Western Blot: Approx 26-28 kDa band observed in Human, Mouse and Rat Skin lysates and in lysates of cell lines A431 and U2OS (calculated MW of 27.8 kDa according to Human NP_006133.1, Mouse NP_061224.2 and Rat XP_003750096.1). Recommended concentration: 0

    Peptide ELISA: antibody detection limit dilution 1:16000.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
    别名
    SFN (SFN 产品)
    别名
    YWHAS antibody, Er antibody, Mme1 antibody, Ywhas antibody, stratifin antibody, SFN antibody, Sfn antibody
    背景
    SFN, 14-3-3sigma, stratifin, HGNC:10773, YWHAS
    基因ID
    2810, 55948, 313017
    NCBI登录号
    NP_006133
    途径
    p53 Pathway, Myometrial Relaxation and Contraction
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