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Fibronectin 1 抗体

This anti-Fibronectin 1 antibody is a 小鼠 单克隆 antibody detecting Fibronectin 1 in IHC, IF, FACS 和 StM. Suitable for 人.
产品编号 ABIN6939413
发货至: 中国

Quick Overview for Fibronectin 1 抗体 (ABIN6939413)

抗原

See all Fibronectin 1 (FN1) 抗体
Fibronectin 1 (FN1)

适用

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宿主

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小鼠

克隆类型

  • 78
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单克隆

标记

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This Fibronectin 1 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

克隆位点

568
  • 特异性

    Epitope of this MAb is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.

    纯化方法

    Purified by Protein A/G

    免疫原

    High molecular weight proteins secreted by cultivated human fibroblasts

    亚型

    IgG1 kappa
  • 应用备注

    Positive Control: SW156 cells. Kidney.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    Fibronectin 1 (FN1)

    别名

    FN1

    分子量

    220kDa (monomer), 440kDa (dimer)

    基因ID

    2335

    UniProt

    P02751

    途径

    Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy
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