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ENO2/NSE 抗体 (AA 416-433)

This anti-ENO2/NSE antibody is a 小鼠 单克隆 antibody detecting ENO2/NSE in WB, IHC 和 StM. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN6939308
发货至: 中国

Quick Overview for ENO2/NSE 抗体 (AA 416-433) (ABIN6939308)

抗原

See all ENO2/NSE (ENO2) 抗体
ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

适用

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人, 小鼠, 大鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This ENO2/NSE antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

克隆位点

ENO2-1375
  • 抗原表位

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    AA 416-433

    特异性

    Recognizes a protein of about 50 kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

    纯化方法

    Purified by Protein A/G

    免疫原

    A synthetic peptide of human NSE gamma (around aa416-433) (exact sequence is proprietary)

    亚型

    IgG2b kappa
  • 应用备注

    Positive Control: HepG2, SH-SY-5Y, HeLa or Y79 cells. Pancreas, Cerebellum or Pheochromocytoma.

    Known Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.2-0.4 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

    别名

    ENO2

    分子量

    ~50kDa

    基因ID

    2026

    UniProt

    P09104
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