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TGFA 抗体 (AA 34-43)

This anti-TGFA antibody is a 小鼠 单克隆 antibody detecting TGFA in IF, FACS 和 IHC (f). Suitable for 人.
产品编号 ABIN6938543
发货至: 中国

Quick Overview for TGFA 抗体 (AA 34-43) (ABIN6938543)

抗原

See all TGFA 抗体
TGFA (Transforming Growth Factor, alpha (TGFA))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This TGFA antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

TG86
  • 抗原表位

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    AA 34-43

    特异性

    This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

    交叉反应 (详细)

    Human. Rabbit. Zebrafish.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGFα.

    亚型

    IgG1 kappa
  • 应用备注

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: Jurkat or Ramos cells. Testicular Carcinoma.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    TGFA (Transforming Growth Factor, alpha (TGFA))

    别名

    TGFA

    背景

    EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1,TGF-alpha (Transforming Growth Factor alpha)
    Cellular localisation: Cytoplasmic and Extracellular (secreted)

    分子量

    ~6kDa

    基因ID

    7039, 170009

    UniProt

    P01135

    途径

    NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
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