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TGFA 抗体 (C-Term)

TGFA 适用: 大鼠 IF, ELISA, IHC (f), Coat 宿主: 小鼠 Monoclonal MF9 unconjugated
产品编号 ABIN6938541
发货至: 中国
  • 抗原 See all TGFA 抗体
    TGFA (Transforming Growth Factor, alpha (TGFA))
    抗原表位
    • 22
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    • 1
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    C-Term
    适用
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    大鼠
    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This TGFA antibody is un-conjugated
    应用范围
    • 51
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    Immunofluorescence (IF), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)
    特异性
    This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
    交叉反应 (详细)
    Human. Cow. Rat. Mouse.
    纯化方法
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    免疫原
    A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGFα (exact sequence is proprietary)
    克隆位点
    MF9
    亚型
    IgG1 kappa
    Top Product
    Discover our top product TGFA Primary Antibody
  • 应用备注

    Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: Jurkat or Ramos cells. Testicular Carcinoma.

    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    储存液
    Azide free
    储存条件
    -20 °C,-80 °C
    储存方法
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    有效期
    24 months
  • 抗原
    TGFA (Transforming Growth Factor, alpha (TGFA))
    别名
    TGFA (TGFA 产品)
    别名
    TFGA antibody, LOC100009482 antibody, TGF alpha antibody, TGFA antibody, wa-1 antibody, wa1 antibody, RATTGFAA antibody, TGFAA antibody, NV15606 antibody, si:ch211-10e8.7 antibody, transforming growth factor alpha antibody, transforming growth factor alpha L homeolog antibody, transforming growth factor-alpha antibody, transforming growth factor, alpha antibody, TGFA antibody, TGF-A antibody, Tgfa antibody, tgfa.L antibody, LOC100121300 antibody, LOC100009150 antibody, tgfa antibody
    背景
    EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1,TGF-alpha (Transforming Growth Factor alpha)
    Cellular localisation: Cytoplasmic and Extracellular (secreted)
    分子量
    ~6kDa
    基因ID
    7039, 170009
    UniProt
    P01135
    途径
    NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
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