TGFA 抗体 (C-Term)
Quick Overview for TGFA 抗体 (C-Term) (ABIN6938541)
抗原
See all TGFA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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特异性
- This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
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交叉反应 （详细）
- Human. Cow. Rat. Mouse.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGFα (exact sequence is proprietary)
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亚型
- IgG1 kappa
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anti-Transforming Growth Factor, alpha (TGFA) (AA 40-89) antibody
TGFA 适用: 人, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 24-98) antibodyTGFA 适用: 人 IHC, WB, IP, ICC 宿主: 小鼠 Monoclonal D20 unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 24-98) antibodyTGFA 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 40-89) antibodyTGFA 适用: 人 IHC, WB, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) antibodyTGFA 适用: 人, 大鼠, 小鼠 IHC, WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) antibodyTGFA 适用: 人, 大鼠, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 118-150) antibodyTGFA 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB56968 unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 1-160) antibodyTGFA 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) antibodyTGFA 适用: 人 WB, ELISA, IHC (p), Func 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (C-Term) antibodyTGFA 适用: 人, 大鼠, 小鼠, Cow IHC, IF, ELISA, StM, Coat 宿主: 小鼠 Monoclonal MF9 unconjugated
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应用备注
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Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat or Ramos cells. Testicular Carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- TGFA (Transforming Growth Factor, alpha (TGFA))
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别名
- TGFA
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背景
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EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1,TGF-alpha (Transforming Growth Factor alpha)
Cellular localisation: Cytoplasmic and Extracellular (secreted) -
分子量
- ~6kDa
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基因ID
- 7039, 170009
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UniProt
- P01135
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途径
- NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
抗原
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