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Melanoma gp100 抗体

PMEL 适用: 人 IF, FACS, IHC (f) 宿主: 小鼠 Monoclonal SPM286 unconjugated
产品编号 ABIN6938387
发货至: 中国
  • 抗原 See all Melanoma gp100 (PMEL) 抗体
    Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This Melanoma gp100 antibody is un-conjugated
    应用范围
    • 39
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    Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    特异性
    By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
    无交叉反应
    大鼠
    交叉反应 (详细)
    Human. Horse. Does not react with Rat.
    纯化方法
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    免疫原
    Membranes from a human melanoma metastasis
    克隆位点
    SPM286
    亚型
    IgG2b kappa
    Top Product
    Discover our top product PMEL Primary Antibody
  • 应用备注

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: SK-MEL-28 cells. Melanoma.

    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    储存液
    Azide free
    储存条件
    -20 °C,-80 °C
    储存方法
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    有效期
    24 months
  • 抗原
    Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
    别名
    SILV (PMEL 产品)
    背景
    95 kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, p100, p26, PMEL17, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SILV, Silver homolog,Melanosome gp100 / PMEL17 / SILV (Melanoma Marker)
    Cellular localisation: Cytoplasmic
    分子量
    90-100kDa
    基因ID
    6490, 95972
    UniProt
    P40967
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