RPA2 抗体
Quick Overview for RPA2 抗体 (ABIN6938326)
抗原
See all RPA2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- Recognizes a protein of 34 kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70 kDa, 32-34 kDa, and 11-14 kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.
-
交叉反应 (详细)
- Human.
-
纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
-
免疫原
- Recombinant human full-length Replication protein A2 (RPA2) protein
-
亚型
- IgG2b kappa
-
-
-
-
应用备注
-
Known_Application: Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7, HeLa or HepG2 cells. Human tonsil (IHC).
-
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
-
储存液
- Azide free
-
储存条件
- -20 °C,-80 °C
-
储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
-
有效期
- 24 months
-
-
- RPA2 (Replication Protein A2, 32kDa (RPA2))
-
别名
- RPA2
-
背景
-
P32, p34, REPA2, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit, Replication protein A2, RF-A protein 2, Rf-A2, RFA, RP-A p32, RP-A p34, RP21C, RPA32, RPA34, RpLP1, RpP2,Replication Protein A2 (RPA2)
Cellular localisation: Nuclear -
分子量
- 32-34kDa
-
基因ID
- 6118, 703070, 79411
-
UniProt
- P15927
-
途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
抗原
-