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POU2AF1 抗体 (AA 148-255)

This anti-POU2AF1 antibody is a 小鼠 单克隆 antibody detecting POU2AF1 in WB, FACS, IF 和 IHC (f). Suitable for 人.
产品编号 ABIN6938224
发货至: 中国

Quick Overview for POU2AF1 抗体 (AA 148-255) (ABIN6938224)

抗原

See all POU2AF1 抗体
POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

适用

  • 79
  • 20
  • 5

宿主

  • 66
  • 28
  • 2
小鼠

克隆类型

  • 64
  • 32
单克隆

标记

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  • 1
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This POU2AF1 antibody is un-conjugated

应用范围

  • 67
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Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

BOB1-2424
  • 抗原表位

    • 23
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    • 1
    • 1
    AA 148-255

    特异性

    BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells.Expression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia.B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.

    交叉反应 (详细)

    Human.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein (exact sequence is proprietary)

    亚型

    IgG2b kappa
  • 应用备注

    Known_Application: Flow Cytometry (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: Raji, Ramos cells. Spleen or Tonsil.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

    别名

    POU2AF1

    背景

    B cell Oct binding protein 1, B-cell-specific coactivator OBF-1, BOB-1, OBF1, OCA-B, OCAB, OCT-binding factor 1, POU domain class 2-associating factor 1, POU2AF1,BOB.1 (B-Cell Marker)
    Cellular localisation: Nuclear

    分子量

    35kDa

    基因ID

    5450, 654525

    UniProt

    Q16633
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