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PODXL 抗体 (Extracellular Domain)

This 小鼠 单克隆 anti-PODXL antibody (Clone 3D3) specifically detects PODXL in WB, IP, FACS, IF 和 IHC (f). The antibody is reactive with 人 samples.
产品编号 ABIN6938203
发货至: 中国
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Quick Overview for PODXL 抗体 (Extracellular Domain) (ABIN6938203)

抗原

See all PODXL 抗体
PODXL (Podocalyxin-Like (PODXL))

适用

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宿主

  • 74
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小鼠

克隆类型

  • 87
  • 47
单克隆

标记

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This PODXL antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

3D3
  • 抗原表位

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    Extracellular Domain

    特异性

    Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

    交叉反应 (详细)

    Human. Rabbit. Rat.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin

    亚型

    IgG1 kappa
  • 应用备注

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunoprecipitation (1-2 μg/500 μg protein), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    PODXL (Podocalyxin-Like (PODXL))

    别名

    PODXL

    背景

    PODXL, PDXL, GCTM-2, Gp200, PCLP1, Pcx, Podocalyxin-like protein 1,Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker)
    Cellular localisation: Cell surface and cytoplasmic

    分子量

    165-170kDa

    基因ID

    5420, 732423

    UniProt

    O00592

    途径

    Tube Formation
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